|
KMID : 0895420130230040333
|
|
Journal of Korean Society of Occupational and Enviromental Hygiene
2013 Volume.23 No. 4 p.333 ~ p.340
|
|
|
Analysis of Asbestos Fiber in Lungs by Transmission Electron Microscopy According to the Digestion Method
|
|
Han Jeong-Hee
Chung Yong-Hyun
Yang Jeong-Sun
|
|
|
Abstract
|
|
|
Objectives: This study was designed to establish an accurate analytical method for asbestos in a biological sample for determining occupational asbestos-related diseases and relief of the health effects of environmental asbestos.
Methods: Biological samples were obtained from lungs of rats following intratracheal instillation of asbestos(Chrysotile, anthophyllite) and were prepared according to digestion method(Wet digestion, high temperature ashing, low temperature ashing). The samples were then analyzed for asbestos fibers using a transmission electron microscope equipped with an energy dispersive X-ray spectrometer.
Results: Low temperature plasma ashing removed more of the organic components and reduced fiber loss compared to the wet digestion method, making specimens so prepared more suitable for transmission electron microsocpy.
Conclusions: The low temperature ashing technique is the most accurate method for analyzing asbestos in biological samples.
|
|
|
KEYWORD
|
|
|
asbestos, lung tissue, digestion, TEM, EDX
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|